Objective: The research aimed to study on the breeding method of the High-yieldGABA(Y- aminobutyric acid) Saccharomyces cerevisiae strain by NTG Mutagenesis. Methods: The strains of Saccharomyces cerevisiae were mutated by NTG,and then were cultured on YEPD medium plate with bromocresol green to screen the mutants. The GABA production in fermentation broth of mutant strains were determined by thin layer chromatography and spectrophotometry. In order to determine the hereditary character of the GABA-producing mutants, after four generations ...