Methamidophos Influences Midgut Proteinase Activity and Subcellular Structures in the Wolf Spider Pardosa pseudoamulata (Araneae: Lycosidae)

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成果类型：

期刊论文

作者：

Wang, Juan;Li, Qinjin;Peng, Yuande;Yang, Huilin;Wang, Zhi*;...

通讯作者：

Wang, Zhi

作者机构：

[Peng, Yuande; Wang, Zhi; Wang, Juan] Chinese Acad Agr Sci, Inst Bast Fiber Crops, Changsha 410205, Hunan, Peoples R China.

[Yang, Huilin; Wang, Juan] Hunan Agr Univ, Coll Biosci & Biotechnol, Changsha 410128, Hunan, Peoples R China.

[Li, Qinjin; Luo, Lin] Hunan Agr Univ, Coll Resources & Environm, Changsha 410128, Hunan, Peoples R China.

[Li, Qinjin] Hunan Womens Univ, Tourism Dept, Changsha 410000, Hunan, Peoples R China.

[Wang, Zhi] Hunan Normal Univ, Coll Life Sci, Changsha 410081, Hunan, Peoples R China.

通讯机构：

[Wang, Zhi] C

[Wang, Zhi] H

Chinese Acad Agr Sci, Inst Bast Fiber Crops, Changsha 410205, Hunan, Peoples R China.

Hunan Normal Univ, Coll Life Sci, Changsha 410081, Hunan, Peoples R China.

语种：

英文

关键词：

insect protein;insecticide;methamidophos;peptide hydrolase;phosphorothioic acid derivative;animal;drug effect;enzymology;female;gastrointestinal tract;hydrolysis;metabolism;spider;ultrastructure;Animals;Female;Gastrointestinal Tract;Hydrolysis;Insect Proteins;Insecticides;Organothiophosphorus Compounds;Peptide Hydrolases;Spiders

期刊：

JOURNAL OF ECONOMIC ENTOMOLOGY

ISSN：

0022-0493

年：

2019

卷：

112

期：

页码：

335-340

DOI：

10.1093/jee/toy318

基金类别：

The authors are most grateful to Prof. Xie Qingji and Dr. Li Yunlong at Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research (Hunan Normal University) for their technical guidance. This work was supported by following sources: Natural Science Foundation of P.R. China (No. 31472017, 31272339). The key projects of Hunan Provincial Science and Technology Department (No. 2014FJ2003). The Planned Science and Technology Project of Hunan Province, China (No. 2015RS4036). The research project of Hunan Provincial Education Department (No. 15C0666). Agricultural Science and Technology Innovation Program of China (No. CAAS-ASTIP-IBFC). The postgraduate research projects of Hunan Province, China (CX2017B361).

机构署名：

本校为其他机构

院系归属：

资源环境学院

生物科学技术学院

摘要：

A piezoelectric quartz crystal impedance (PQCI) sensor was used to investigate influences of the insecticide methamidophos on proteinase activity in midguts of the wolf spider, Pardosa pseudoamulata (Araneae: Lycosidae). Results from PQCI indicated that low-concentration dose methamidophos (0.008%) can activate the proteinase but high-concentration dose methamidophos (0.016-0.032%) can inhibit the enzyme activity. The changes in subcellular structure of spider midgut cells were also observed. Electron micrographs of spider midgut epithelial cel...

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Methamidophos Influences Midgut Proteinase Activity and Subcellular Structures in the Wolf Spider Pardosa pseudoamulata (Araneae: Lycosidae)

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