期刊论文

Zhang, Heng-Mu

英文

Gene

0378-1119

2014

548

2

285-293

10.1016/j.gene.2014.07.048

This work was funded by the National Science and Technology Support Program ( 2012BAD19B03 ), the China 973 Program ( 2006CB708209 ), the 863 Program ( 2007AA10Z414 ), the Special Fund for Agro-scientific Research in the Public Interest of China ( 201003031 ), the Zhejiang Provincial Science and Technology Project ( 2010C12027 ), and the Zhejiang Provincial Foundation for Natural Science ( Z305165 and Y3090657 ). We thank Dr Zhiyou Du, School of Life Science, Zhejiang Sci-Tech University, for the technological help and Professor M. J. Adams, Rothamsted Research, Harpenden, UK for his help in correcting the English of the manuscript.

本校为其他机构

Plant microRNAs (miRNAs) play important roles as modulators of gene expression at the post-transcriptional level. Previous studies have shown that high-throughput sequencing is a powerful tool for the identification of miRNAs, and it is believed that many more miRNAs remain to be discovered. Here, we found 23 novel conserved miRNAs from three rice cultivars by high-throughput sequencing and further identified these through subsequent cloning and quantitative real-time polymerase chain reaction (qPCR). Eight of these novel miRNAs were detected with significant signals in the three rice cultivar...