This study was designed to explore the toxic effects of cadmium on piglet sertoli cells. Sertoli cells were isolated from piglet testes using a two-step enzyme digestion, followed by differential plating and were identified by oil red O staining and immunocytochemical methods. Piglet sertoli cells were cultured and classified into five groups, i. e, control group without cadmium chloride; and 4 separate groups exposed to 10, 20, 40 and 80 μmol · L-1 cadmium chloride. We found that highly purified sertoli cells were isolated by the two-step en...
