期刊论文

Wang, H.

中文

环境科学学报

0253-2468

2010

30

2

327-339

10.13671/j.hjkxxb.2010.02.004

国家自然科学基金项目（No.50708120）； 国家高技术研究发展计划（863）项目（No.2004AA649320）~~；

本校为第一机构

采用电镜观察和分子生物学手段16S rDNA克隆文库方法对亚硝化/电化学生物反硝化全自养脱氮工艺中的细菌进行了形态和多样性研究,从16S rDNA克隆文库中随机挑选60(61)个克隆子进行序列测定(约500bp),对测序结果进行BLAST比对和系统发育分析.结果表明,亚硝化段内的细菌主要为球状和椭球状的氨氧化细菌,以亚硝酸氮作为进水基质时,电化学反硝化生物段内细菌主要为短杆状和椭球状的脱氮菌.亚硝化/电化学生物反硝化脱氮系统中蕴藏着特有的微生物新资源.亚硝化段细菌类群的优势顺序为β-Proteobacteria类群(60.00%)、Bacteroidetes类群(28.33%)和Chloroflexi类群(11.67%).当电化学生物反硝化段进水氮基...

The morphology and diversity of the bacteria in the complete autotrophic nitrlte-nitrification and electrochemical bio-denitrification combined process were studied by scanning electron microscopy (SEM) and molecular biological methods, I.e. Partial 16S rDNA cloning and sequencing. Sixty (61) clones were randomly selected, and their partial 16S rDNA gene (ca. 5006p) was sequenced and searched in the BLAST database. A representative clone of each phylotype was also phylogenetically analyzed. The dominant bacteria in the nitrite-nitrification pha...