期刊论文

Lu, X.

中文

环境科学学报

0253-2468

2011

31

3

492-498

10.13671/j.hjkxxb.2011.03.006

国家“十一五”科技支撑计划（No. 2008BADC4B08）； 科技部国际科技合作项目（No. 2010DFA62510）； 研究生创新基金项目（No.54040108003）~~；

本校为第一且通讯机构

以从土壤中分离得到的黑曲霉(Aspergillus niger)菌株为研究对象,采用液体培养的方法,研究其在不同碳源,氮源,起始pH值,金属离子种类及浓度条件下对孟加拉红和水溶性色浆的脱色效果.结果表明,在以豆芽汁培养基为基本培养基,甘油为碳源,(NH4)2SO4为氮源,初始pH值为6.0~8.0条件下的脱色效果最佳, Cu2+对菌体脱色有很强的抑制作用.根据活菌体和死菌体脱色能力的比较,结合菌体干重与脱色能力的相关性分析,初步推测该菌的脱色机理是菌丝吸附染料后,在菌丝体内对其进行降解;降解过程有限速酶参与,柠檬酸钠,酒石酸钠会抑制该酶的活性,而NH4+对该酶有激活作用.关键词:黑曲霉;孟加拉红;色浆;脱色机理

Aspergillus niger isolated from soil by enrichment culture was chosen as the research object in this paper. The decoloring properties of Aspergillus niger to rose bengal and water-soluble color paste were studied in liquid culture with different carbon and nitrogen sources, pH value, and added metal ions. The decoloring effect was the best in a medium of bean sauce, with glycerol as carbon source, (NH 4 ) 2 SO 4 for nitrogen source, and initial pH of 6.0~8.0, but Cu 2+ obviously inhibited the activity. By comparing the decoloring ability of living mycelium and dead mycelium, combined with corr...