期刊论文

Effects of Long-term Fertilization on Enzyme Activities in Profile of Paddy Soil Profiles

Zhu, Zhen-Ke(zhuzhenke@isa.ac.cn)

中文

环境科学

0250-3301

2018

39

8

3901-3909

10.13227/j.hjkx.201711234

国家重点研发计划项目（2016YFD0300902）； 国家自然科学基金项目（41501321）； 湖南省自然科学基金项目（2016JJ3132,2016JJ2136）； 农业部长江中游平原农业环境重点实验室开放基金项目；

本校为第一机构

与稻田土壤碳周转密切相关的酶活性是评价土壤肥力和肥料管理的重要指标.本研究选取秸秆还田(ST) 、化肥(NPK)和不施肥(CK)的长期定位试验田,以10 cm的间距分段采集土壤剖面0 ~ 40 cm范围内的新鲜土样,利用96微孔酶标板荧光分析法,测定参与土壤碳氮转化过程关键酶β-1,4-葡萄糖苷酶(BG)和β-1,4 -N-乙酰氨基葡萄糖苷酶(NAG)活性,探讨不同施肥措施对不同深层土壤酶活的影响.结果表明,相比不施肥的土壤,施用化肥和秸秆土壤的BG酶活性分别提高了35% ~ 118%和55% ~ 342%; NAG酶活性分别提高了9% ~ 30%和102% ~ 484%.同时,随着土层深度的增加,土壤酶活性逐渐降低,0 ~ 20 cm深层土壤酶活性显著高于20 ...

The enzyme activity, which is closely related to soil material cycling (mineralization, transformation, etc.), can reflect soil quality and nutrient status. In order to explore the effect of long-term fertilization on the enzyme activity in paddy soil profile (0-40 cm), soils with organic fertilizer and inorganic fertilizer, and non-fertilized soils were selected, and the carbon and nitrogen contents, and the activities of β-1,4-glucosidase (BG), and β-1,4-N-acetylglucosaminidase (NAG) in 10cm depths of soil were analyzed. The results showed ...