期刊论文

中文

家畜生态学报

1673-1182

2019

40

1

13-17

10.3969/j.issn.1673-1182.2019.01.003

31401097:国家自然科学基金 2013AA102503:国家高技术研究发展计划（863计划） 2016NK2169:湖南省重点研发计划项目 2017NK2331:湖南省重点研发计划项目 2017TP1030:湖南省重点实验室开放研究项目

本校为第一机构

以沙子岭猪、大约克、巴克夏猪及其杂交组合巴×沙、大×巴沙猪为研究对象,测定背最长肌中肌苷酸含量,对ADSL、GPAT和PURH基因在不同群体背最长肌中的表达水平进行检测,分析其与肌苷酸含量的相关性.结果表明,沙子岭猪肌苷酸含量显著高于巴克夏和大×巴沙猪(P＜0.05),其余各群体之间差异均不显著(P＞0.05).肌苷酸含量与肌肉pH1 h、肉色L*值和a*值、肌内脂肪含量、剪切力等肉质指标具有显著相关性.ADSL、GPAT和PURH基因在不同群体中的表达模式基本相同,即三种基因均在沙子岭猪和巴×沙猪中高表达.ADSL基因表达量与肌苷酸含量呈极显著正相关(P＜0.01),GPAT和PURH基因表达量与肌苷酸含量均呈显著正相...

In this research,Shaziling,Yorkshire and Berkshire,and their crossbreeds Berkshire×Shaziling,Yorkshire×Berkshire×Shaziling pigs is chosen as the study objects.The inosine monophosphate(IMP)content in longissimus dorsi muscle was measured by HPLC.Meanwhile,the relative expressions of ADSL,GPAT and PURH genes in different groups were detected by real-time PCR,and the correlations between expressions of genes and IMP content were analyzed.The results demonstrate that the IMP content in Shaziling pigs is significantly higher than that in Berkshi...