期刊论文

Improvements on methods of Agrobacterium-mediated genetic transformation of Indica rice

中文

长沙理工大学学报(自然科学版)

1672-9331

2008

5

3

98-103

10.3969/j.issn.1672-9331.2008.03.017

教育部科学技术研究重点项目（204101）；

本校为其他机构

采用培矮64S和9311两种籼稻为材料,研究了影响农杆菌介导转化的几种因素.研究结果表明,预培养4 d的幼胚最适宜作为农杆菌介导转化的受体,其次是来源于幼胚和成熟胚的生长状态良好的胚性愈伤组织.以AAM培养基作为共培养培养基.对于农杆菌EHA105(pUblm)来说,菌液浓度为OD600值=0.8时.愈伤组织的转化率最高.在农杆菌侵染愈伤组织过程中,通过负压处理可明显提高愈伤组织的转化频率.在愈伤组织浸染后,以含一定浓度的Ca2+和表面活性剂Tween20的溶液处理可明显提高水稻愈伤组织的转化频率.

Several factors that influence transformation frequency of two varieties of indica rice Pei＇ai64S and 93 llmediated by Agrobaterium tumefaciens were investigated. The results indicated that immature embryos after being precultured for 4d were the best transformation acceptor. In respect to the concentration of Agrobacterium tumfaciences when calli were cotransformated in medium AAM,to Agrobacterium tumfaciences EHA 105, OD600 value of 0. 8 is the best. Negative pressure disposition in the process of Agrobacterium tumfaciences infecting callus ...