Molecular cloning and functional identification of a novel borneol dehydrogenase from Artemisia annua L.

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成果类型：

期刊论文

作者：

Tian, Na;Tang, Yuwei;Xiong, Shuo;Tian, Dongming;Chen, Yuhong;...

通讯作者：

Liu, Shuoqian

作者机构：

[Tian, Na; Liu, Zhonghua; Liu, Shuoqian] Hunan Agr Univ, Hunan Prov Key Lab Germplasm Innovat & Utilizat C, Hunan Collaborat Innovat Utilizat Bot Funct Ingre, Coll Hort & Hardening, Changsha 410128, Hunan, Peoples R China.

[Tian, Na; Tian, Dongming; Liu, Zhonghua; Liu, Shuoqian; Tang, Yuwei] Natl Res Ctr Engn Technol Utilizat Funct Ingredie, Changsha, Hunan, Peoples R China.

[Xiong, Shuo; Wu, Dunchao; Liu, Zhonghua; Liu, Shuoqian; Chen, Yuhong] Minist Educ, Key Lab Tea Sci, Changsha 410128, Hunan, Peoples R China.

通讯机构：

[Liu, Shuoqian] H

Hunan Agr Univ, Hunan Prov Key Lab Germplasm Innovat & Utilizat C, Hunan Collaborat Innovat Utilizat Bot Funct Ingre, Coll Hort & Hardening, Changsha 410128, Hunan, Peoples R China.

语种：

英文

关键词：

Artemisia annua L;Borneol;Borneol dehydrogenase;Camphor

期刊：

Industrial Crops and Products

ISSN：

0926-6690

年：

2015

卷：

页码：

190-195

DOI：

10.1016/j.indcrop.2015.08.049

基金类别：

We sincerely thank Dr. Weiping Zhu (Hunan Agricultural University, Changsha, China) for providing plant material. This work was financially supported by the International Science & Technology Cooperation Program of China ( 2014DFA32160 ) and the National Science Foundation of China (No. 31270335 ).

机构署名：

本校为第一且通讯机构

院系归属：

园艺园林学院

摘要：

In order to investigate the physiological functions and biosynthesis regulation of borneol or comphore in Artermisia annua L., which is the major source of the anti-malaria drug artemisinin, the full length cDNA of the gene encoding a borneol dehydrogenase (AaBDH) was cloned from A. annua for the first time by using RACE (rapid amplification of cDNA ends). The completed open read frame of AaBDH was 1415 bp and it encoded a 885-amino acid protein with a predicted molecular mass of 31.04 kDa and a pI of 6.16. AaBDH showed 68-70% of amino acid identity to alcohol dehydrogenases from Solanum lycop...

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Molecular cloning and functional identification of a novel borneol dehydrogenase from Artemisia annua L.

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