Flax is an important economic crop for fiber and/or oil in China. An efficient flax plant regeneration system was established through optimization of basal medium, hormone combination, flax genotype and age of hypocotyl explants. The best medium for callus and adventitious shoot differentiation was YB1. Shoot induction rate of Baihua, Heiya NO.4, K6531, K7697, HI026, HI045, 1039 and Aliyana was 98.50%, 98.50%, 56.50%, 42.47%, 54.40%, 0, 27.13%, and 97.30%, respectively, and mean shoot number per explant was 11.43, 9.33, 2.17, 0.77, 1.10, 0, 0.90, and 10.68, respectively. Hypocotyls taken from ...