期刊论文

Qu, SH

英文

植物学报：英文版

1672-9072

2009

51

11

982-992

10.1111/j.1744-7909.2009.00870.x

Acknowledgements We are grateful to Chatchawan Jantasuriyarat and Bo Zhou for experimental assistance, to David Ow （Plant Gene Expression Center, USDA, Albany, CA 94710, USA） for providing the Cre gene, and to Yoshihito Suzuki （Department of Applied Biological Chemistry, University of Tokyo, Tokyo 113-8657, Japan） for the DNA construct containing the 4x CaMV 35S enhancers.

本校为其他机构

Transposons are effective mutagens alternative to T-DNA for the generation of insertional mutants in many plant species including those whose transformation is inefficient. The current strategies of transposon tagging are usually slow and labor-intensive and yield low frequency of tagged lines. We have constructed a series of transposon tagging vectors based on three approaches: (i) AcTPase controlled by glucocorticoid binding domain/VP16 acidic activation domain/Gal4 DNA-binding domain (GVG) chemical-inducible expression system; (ii) deletion of AcTPase via Cre-lox site-specific recombination...