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Genomics insights into different cellobiose hydrolysis activities in two Trichoderma hamatum strains

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成果类型:
期刊论文
作者:
Cheng, Peng*;Liu, Bo;Su, Yi;Hu, Yao;Hong, Yahui;...
通讯作者:
Cheng, Peng;Chen, Nansheng;Chu, Jeffrey S. C.
作者机构:
[Su, Yi; Chen, Lei; Xiong, Xingyao; Cheng, Peng; Hong, Yahui; Su, Shengying] Hunan Agr Univ, Hunan Prov Key Lab Phytohormones & Growth Dev, Hunan Prov Key Lab Crop Germplasm Innovat & Utili, Changsha 410128, Hunan, Peoples R China.
[Chen, Nansheng; Liu, Bo; Hu, Yao; Yi, Xinxin] Huazhong Agr Univ, Coll Life Sci & Technol, Wuhan 430070, Peoples R China.
[Cheng, Peng] Hunan Agr Univ, Natl Ctr Citrus Improvement, Changsha 410128, Hunan, Peoples R China.
[Chu, Jeffrey S. C.] Wuhan Frasergen Bioinformat Co Ltd, East Lake Hightech Zone, 666 Gaoxin Rd, Wuhan 430075, Peoples R China.
[Chen, Nansheng] Simon Fraser Univ, Dept Mol Biol & Biochem, Burnaby, BC V5A 5S6, Canada.
通讯机构:
[Cheng, Peng; Chen, Nansheng] H
[Chu, Jeffrey S. C.] W
[Chen, Nansheng] S
Hunan Agr Univ, Hunan Prov Key Lab Phytohormones & Growth Dev, Hunan Prov Key Lab Crop Germplasm Innovat & Utili, Changsha 410128, Hunan, Peoples R China.
Huazhong Agr Univ, Coll Life Sci & Technol, Wuhan 430070, Peoples R China.
语种:
英文
关键词:
beta glucosidase;cellobiose;cellulose;enzyme GH1;enzyme GH18;enzyme GH3;enzyme GH35;enzyme GH55;exoglucanase;fungal enzyme;glucan synthase;proteinase;unclassified drug;beta glucosidase;biofuel;cellobiose;cellulase;peptide hydrolase;Article;CAZyme gene;controlled study;degradation kinetics;enzyme activity;expression vector;fungal gene;fungal genome;fungal strain;gene sequence;genome analysis;genomics;hydrolysis kinetics;nonhuman;pGEX KG gene;protease gene;synteny;Trichoderma;Trichoderma hamatum;YYH1311079 gene;biomass;biosynthesis;DNA sequence;enzymology;fermentation;genetic variation;genetics;genomics;hydrolysis;metabolism;Trichoderma;beta-Glucosidase;Biofuels;Biomass;Cellobiose;Cellulase;Cellulose;Fermentation;Genetic Variation;Genome, Fungal;Genomics;Hydrolysis;Peptide Hydrolases;Sequence Analysis, DNA;Trichoderma
期刊:
Microbial Cell Factories
ISSN:
1475-2859
年:
2017
卷:
16
期:
1
页码:
1-16
基金类别:
The present study was supported by National Natural Science Foundation of China (31570372), Scientific research outstanding youth project of Hunan Provincial Education Department (15B112), Science and technology project of Hunan Province (2015NK3005), The Natural Science foundation of Hunan Province (2016JJ3074), Science and technology project of Changsha City (kh1601179), The Open Science Foundation of Hunan Provincial Key Laboratory for Germplasm Innovation and Utilization of Crop (15KFXM13).
机构署名:
本校为第一且通讯机构
院系归属:
园艺园林学院
生物科学技术学院
摘要:
Background: Efficient biomass bioconversion is a promising solution to alternative energy resources and environmental issues associated with lignocellulosic wastes. The Trichoderma species of cellulolytic fungi have strong cellulose-degrading capability, and their cellulase systems have been extensively studied. Currently, a major limitation of Trichoderma strains is their low production of β-glucosidases. Results: We isolated two Trichoderma hamatum strains YYH13 and YYH16 with drastically different cellulose degrading efficiencies. YYH13 has...

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