期刊论文

中文

动物学报

1674-5507

2007

53

1

184-189

10.3969/j.issn.1674-5507.2007.01.023

30571414:国家自然科学基金 06JJ20056:湖南省自然科学基金 06C165:湖南省社会科学基金

本校为其他机构

微卫星(Microsatellite)是一类由2-6个核苷酸经多次单位串联组成的高度变异重复DNA序列(Schlotterer and Tautz,1992).它具有按照孟德尔方式分离、突变快、多态信息含量丰富、呈共显性遗传等特点,其核心序列在同一物种中具有保守性,因此,可以根据微卫星的侧翼序列设计合适的引物对其核心序列进行PCR扩增.

This project made use of bioinformatic mining of microsatellites from genomic resources to identify simple sequence repeats （SSRs）, or microsatellites. A bioinformatic analysis of 304 nucleotide sequences in Siniperca chuatsi GenBank identified 22 sequences containing 30 microsatellites, account for 9.87% of wholly GenBank database. Cluster analysis indicated that 16 dinucleotide pairs were the most abundant microsatellites, accounting for 53.33 % of the total microsatellite-containing sequences; 11 trinucleotide repeats and 3 tetranucleotide...