In the paper, the research purpose is selecting heat-tolerance molecular markers from Holstein cattle and applying it to the research that helps to establish high-yield and high heat tolerance dairy cattle breed. With 10 high yield and different heat-tolerance Holstein Cattle as materials, we was optimized RAPD reaction system, and 64 primers with better result were selected in 154 random primers. Considered the detected result of potassium content in erythrocyte and production performance, three molecular markers relative to h...