期刊论文

Detection of the Heat-resistant RAPD-S463 Marker in Holstein

中文

中国奶牛

1004-4264

2009

3

9-11

10.3969/j.issn.1004-4264.2009.03.003

国家科技支撑计划(2006BAD04A01) 湖南省科技厅(05NK2004) 长沙市科技局(K052027-221)

本校为第一机构

本试验以S463为奶牛假定抗热应激的RAPD分子标记,在核心母牛场湖南省奶牛原种场测定不同世代的种母牛213头,在核心公牛场湖南省种公牛站,以及北京、上海、南京、安徽等地测定种公牛46头,共检测出具有S463分子标记的母牛26头,检出率为12.2%(26/213),公牛10头,检出率为21.7%(10/46).在湖南省奶牛原种场,有、无抗热应激分子标记的母牛在热应激期间的产奶量分别为1 724.53kg和1 531.62kg,产奶量下降率分别为3.47%和23.18%,产奶量差异不显著(P>0.05),但产奶量下降率差异显著(P

S463 marker is a suppositive heat-resistant RAPD molecular marker. For further research, 213 dairy cows which from different generations had been determined in Hunan foundation seed farm and 46 bulls had been determined in the core bull station of Beijing, Shanghai, Nanjing, Anhui. The result showed that 26 dairy cows and 10 bulls had S463 marker, the detection rates were 12.2%(26/213) and 21.7%(10/46) respectively. In Hunan foundation seed farm, the ...