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Selection of reference genes for expression analysis using RT-qPCR in the dissemination system of Heliothis virescens ascovirus 3 h (HvAV-3h)

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成果类型:
期刊论文
作者:
Chen, Zi-Shu;Han, Ning-Ning;Li, Jian-Hong;Huang, Guo-Hua;Wan, Hu*
通讯作者:
Wan, Hu
作者机构:
[Chen, Zi-Shu; Li, Jian-Hong; Wan, Hu; Han, Ning-Ning] Huazhong Agr Univ, Coll Plant Sci & Technol, Hubei Insect Resources Utilizat & Sustainable Pes, Wuhan 430070, Hubei, Peoples R China.
[Chen, Zi-Shu; Huang, Guo-Hua] Hunan Agr Univ, Inst Virol, Coll Plant Protect, Changsha 410128, Hunan, Peoples R China.
通讯机构:
[Wan, Hu] H
Huazhong Agr Univ, Coll Plant Sci & Technol, Hubei Insect Resources Utilizat & Sustainable Pes, Wuhan 430070, Hubei, Peoples R China.
语种:
英文
期刊:
Scientific Reports
ISSN:
2045-2322
年:
2017
卷:
7
期:
1
页码:
7045
基金类别:
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31371995]; Hunan Provincial Natural Science Foundation for Distinguished Young Scholars of China [14JJ1023]; National Key Technology R&D Program of ChinaNational Key Technology R&D Program [2012BAD27B02]
机构署名:
本校为其他机构
院系归属:
植物保护学院
摘要:
Ascoviruses are double-stranded DNA viruses that mainly infect noctuid larvae, and are transmitted by the parasitoid wasp Microplitis similis Lyle. Ascovirus-parasitoids wasp-noctuid insects constitute the dissemination system. Selection of suitable reference genes for the dissemination system could play an important role in elucidating the pathogenic molecular mechanisms of ascovirus. Unfortunately, such studies on potential reference genes in the dissemination system of ascoviruses are lacking. In the present study, we evaluated 11 candidate reference genes: beta-actin1 (ACT1), beta-actin2 (...

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