Improved yield of theaflavin-3,3ʹ-digallate from Bacillus megaterium tyrosinase via directed evolution

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成果类型：

期刊论文

作者：

Zhou, Jinghui;Liu, Changwei;Zhao, Shimin;Liu, Ya;Zhang, Sheng;...

通讯作者：

Liu, Zhonghua

作者机构：

[Liu, Changwei; Liu, Zhonghua; Zhang, Sheng; Zhou, Jinghui; Huang, Jianan] Hunan Agr Univ, Minist Educ, Key Lab Tea Sci, Changsha 410128, Peoples R China.

[Liu, Changwei; Liu, Zhonghua; Zhang, Sheng; Zhou, Jinghui; Huang, Jianan] Hunan Agr Univ, Natl Res Ctr Engn & Technol Utilizat Bot Funct In, Changsha 410128, Peoples R China.

[Liu, Changwei; Liu, Zhonghua; Zhang, Sheng; Zhou, Jinghui; Huang, Jianan] Hunan Agr Univ, Educ Minist Utilizat Bot Funct Ingredients, Coinnovat Ctr, Changsha 410128, Peoples R China.

[Zhao, Qiang; Wang, Fen; Zhao, Shimin; Xu, Gang; Liu, Ya] Hunan Flag Biotechnol Co Ltd, Changsha 410100, Peoples R China.

通讯机构：

[Liu, Zhonghua] H

Hunan Agr Univ, Minist Educ, Key Lab Tea Sci, Changsha 410128, Peoples R China.

语种：

英文

关键词：

Directed evolution;Theaflavin-3,3ʹ-digallate;Theaflavins;Tyrosinase

期刊：

Food Chemistry

ISSN：

0308-8146

年：

2022

卷：

375

页码：

131848

DOI：

10.1016/j.foodchem.2021.131848

基金类别：

This work was supported by the China Agriculture Research System of MOF and MARA, Post Scientist of National Modern Agricultural Tea Industry Technology System---Utilization of Functional Ingredients (No. CARS-23); The Key R&D Program of Hunan Province (2020NK2047 & 2020NK2030 & 2020WK2017).

机构署名：

本校为第一且通讯机构

院系归属：

园艺园林学院

摘要：

Theaflavin-3,3ʹ-digallate (TFDG) in black tea possesses several health benefits. However, low TFDG yields limit its application. Herein, tyrosinases from Bacillus megaterium (Bmtyrc) were used to synthesize TFDG. To improve the catalytic efficiency of tyrosinase, a directed evolution strategy and a high-throughput screening method was employed. Compared with the wild type, mutant Bmtyrc-3 (N205D/D166E/D167G/F197W) showed 6.46 and 4.91-folds higher specific activity and 51.97- and 1.95-folds higher Vmax values towards epigallocatechin gallate (...

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Improved yield of theaflavin-3,3ʹ-digallate from Bacillus megaterium tyrosinase via directed evolution

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