In order to clarify the resistance level and resistance mechanism to cyhalofop-butyl in Echinochloa crus-galli populations from Hunan and Jiangxi paddy fields,the experimental studies were carried out by whole-plant bioassay,gene sequencing,and metabolic enzyme inhibitor coupled with cyhalofop-butyl.The results showed that R1,R2 and R3 populations displayed medium level resistance to cyhalofop-butyl,with the resistance indexs of 3.08,2.19 and 3.83,respectively.R4 population displayed low level resistance to cyhalofop-butyl,with the resistance index of 1.45.The Ile-2041-Val mutation was present...