期刊论文

Construction of Mutant Population and Analysis of Dwarf Mutants in ＂6421＂ （Capsicum annuum L.） through EMS Mutagenesis

中文

农业科学与技术(英文版)

1009-4229

2016

17

6

1322-1325+1408

10.16175/j.cnki.1009-4229.2016.06.010

Supported by Natural Science Foundation of Hunan（2016JJ6064）； China Agriculture Research System（CARS-25-A-8）~~；

本校为其他机构

[目的]为促进辣椒的遗传改良,以“6421”为试验材料,建立辣椒突变体库。[方法]采用不同浓度的EMS溶液（0.2%～1.2%）处理“6421”种子,确定半致死剂量,并构建突变体,并对M4代中获得的矮秆突变体进行农艺性状调查与遗传分析。[结果]EMS浓度为1.0%时,种子发芽率和出苗率分别为45.2%和40.2%,种子发芽指数和生活力分别为17.6和19.7,接近半致死;取经半致死浓度处理的种子10,000粒构建突变体,2015年在M_4代中共获得562份能稳定遗传的突变体,突变性状可分为11大类32小类;矮秆突变体E29,E58,E142和E312的株高、株幅、主茎粗、主茎长以及主茎节位数均显著低于对照植株,E58侧枝数显著高于对照植株;E29,E58...

[Objective] The aim was to improve the genetic property of peppers, the mutant population of Capsicum annuum L cultivar ＂6421＂ was constructed. [Method] The seeds of ＂6421＂ were treated with 0.2% to 1.2% ethyl methane sulfonate to identified LD50, and then 10 000 LD~o of treated seeds were sowed to construct mutant population. The agronomic characters and genetic regularity of dwarf mutants in M4 generation were analyzed. [Result] Our results showed that GR and SSR were 45.2% and 40.2% respectively at 1.0% EMS, close to LD50, with GI （17.6...