期刊论文

Analysis of Caffeoylquinic Acids in Sweetpotato by HPLC

中文

农业科学与技术(英文版)

1009-4229

2016

17

12

2734-2737+2741

10.16175/j.cnki.1009-4229.20161216.002

Supported by Postdoctoral Sustentation Fund of Hunan Province（2014RS4010）； the Open Fund for Key Horticulture Discipline Research of Hunan Agricultural University（2013YYX021）~~；

本校为第一机构

[目的]运用HPLC法比较分析11种甘薯不同部位绿原酸含量，为甘薯尤其是地上部分茎叶的开发利用提供参考。[方法]样品通过质量为200倍的甲醇超声提取20 min，乙腈-0.2%磷酸为流动相，梯度洗脱，对绿原酸、4,5-O-咖啡酰基奎尼酸、3,5-O-咖啡酰基奎尼酸、3,4-O-咖啡酰基奎尼酸形成良好分离，加标回收率分别为：94.74%、102.63%、100.01%和101.05%。[结果]不同甘薯品种间及同一品种不同部位间咖啡酰奎尼酸类化合物含量显著差异；地上部分以双咖啡酰奎尼酸含量为主，块薯中以绿原酸含量最高；各组织部位咖啡酰奎尼酸类化合物总量分别为：茎尖9.77～40.86 mg/g，成熟叶2.68～13.97 mg/g，茎干0.56～7.9...

[Objective] An HPLC method for the determination of caffeoylquinic acid compounds in sweet potato was developed in the present research. Quantitative analysis was performed on 11 sweet potato genotypes to provide a reference for the development and utilization of sweet potato, especial y on the aboveground part. [Method] Caffeoylquinic acid compounds were extracted from sweet potato leaves by ultrasonic treatment for 20 min with 200 volumes of methanol. The mobile phase of HPLC was acetonitrile -0.2% aqueous phosphoric acid. Chlorogenic acid, 4...