期刊论文

中文

Agricultural Science & Technology

1009-4229

2019

20

06

28-35

10.16175/j.cnki.1009-4229.2019.06.004

Supported by National Key Research&Development Plan （2018YFD0201204）~~

本校为第一机构

以通道侗族自治县当地栽培种生姜的幼芽为外植体,研究了生姜的组培快繁技术,并对获得的生姜组培苗进行了CMV（黄瓜花叶病毒）和TMV（烟草花叶病毒）检测。结果表明:生姜幼苗增殖诱导分化的适合培养基为MS+6-BA 4.0 mg/L+NAA 0.2 mg/L,在此培养基上可实现生姜幼苗增殖与生根的同步培养以及一步炼苗与移栽,增殖倍数达到3.00;适合的生根培养基为1/2MS+0.4 mg/L NAA;生姜炼苗移栽4种栽培基质中以椰糠和蛭石的成活率最高,达到100%,草炭和棉籽壳的成活率很低,其中蛭石移栽的生姜幼苗较椰糠移栽的幼苗植株生长更健壮,根系发达且不定根较多,叶片黄化较轻;生姜组培苗的病毒检测发现均不含CMV和TMV这2种病...

The buds of ginger grown in Tongdao Dong Autonomous County were used as explants, the tissue culture and rapid propagation of ginger were studied and the CMV (Cucumber mosaic virus) and TMV (Tobacco mosaic virus) of ginger tissue culture seedlings were detected. The results showed that MS+6-BA 4.0 mg/L+NAA 0.2 mg/L was the suitable medium for the proliferation induction and differentiation of ginger seedlings. This medium can realize the synchronous culture of ginger seedling proliferation and rooting, and one-step seedling reifning and transpl...