期刊论文

中文

热带作物学报

1000-2561

2008

29

5

622-625

10.3969/j.issn.1000-2561.2008.05.017

广东省农业科学院院长基金项目（04-基金-04B）资助；

本校为第一机构

研究荔枝(Litchi chinesis Sonn)胚性悬浮细胞玻璃化法超低温保存的一些因素,建立玻璃化法超低温保存荔枝胚性悬浮细胞的冻存程序:继代培养3 d的胚性悬浮细胞,在含有O.4 mol/L山梨醇培养基上预培养2 d后,先在室温下用60%玻璃化溶液(PVS2)装载15 min,再用PVS2于0℃下处理15～20 min,换1次新鲜的PVS2溶液后.迅速投入液氮,保存24 h后,在40℃水浴中快速化冻,用1.2 mol/L蔗糖培养液洗涤2次,经TTC检测,相对存活率达到27.1%.

Embryogenic suspension cells from Litchi （Litchi chinesis Sonn ） was vitrified and then cryopreserved. Three days old embryogenic suspension cells were precultured for 2 days at 25% on MS medium supplemented with 0.4mol/L glucitol, and then loaded in a vitrification solution containing 60% PVS2 for 15 minutes at room temperature. The embryogenic suspension ceils were sufficiently dehydrated with vitrification solution containing 100% PVS2 for 15-20 minutes at 0℃. After the solution was changed with fresh PVS2, the suspensions were immersed i...