期刊论文

Cloning and Expression Analysis of Mevalonate Diphosphate Decarboxylase Gene in Hedera helix L

中文

热带作物学报

1000-2561

2018

39

11

2200-2206

10.3969/j.issn.1000-2561.2018.11.013

No.1630032018006:中央级公益性科研院所基本科研业务费专项 No.15A089:湖南省教育厅科学研究项目

本校为第一机构

甲羟戊酸焦磷酸脱羧酶(Mevalonate diphosphate decarboxylase,MVD)是三萜皂苷生物合成途径中的关键酶,为研究其在洋常春藤中的基因功能,通过RT-PCR和RACE技术从洋常春藤叶片中克隆获得HhMVD基因的cDNA全长序列,并通过RT-qPCR技术分析其表达规律.结果表明:RACE克隆获得的HhMVD基因cDNA序列全长1 799 bp,包含一个完整开放阅读框(ORF)1 263 bp、5'非编码区(5'UTR)192 bp、3'非编码区(3'UTR)344 bp;该基因编码420个氨基酸,分子质量46.6 ku,理论等电点为6.57,不含跨膜区,属于非分泌型蛋白;HhMVD蛋白具有GHMP激酶N端结构域,属于甲羟戊酸激酶系列,与刺五加、人参、三七等同科植物亲缘关系较近.RT-qPCR...

Mevalonate diphosphate decarboxylase is a key enzyme in the biosynthesis pathway of triterpenoid saponins. In order to analyze its gene function, the full-length cDNA sequence was cloned successfully by RT-PCR and RACE methods in the leaves of H. helix, the expression trends were illustrated through RT-qPCR analysis. Results indicated that the cDNA sequence of HhMVD was 1 799 bp which contained a ORF from 193 bp to 1 455 bp and a 5'UTR with 192 bp and a 3'UTR with 344 bp. It encoded a 420-amino-acid protein with a molecular weight of 46.6 ku and an isoelectric point (pI) of 6.57. The HhMVD pro...