期刊论文

Selection and Comprehensive Evaluation of Cassava Cold-resistant Germplasm Resources and Identification Indexes

中文

热带作物学报

1000-2561

2019

40

1

1-10

10.3969/j.issn.1000-2561.2019.01.001

现代农业产业技术体系建设专项资金资助项目(No.CARS-11-HNSY) 湖南省重点研发计划资助项目(No.2017NK2054)。

本校为第一机构

先利用田间自然低温对18个木薯种质资源进行冷害分级,从中选择强耐寒品种(F200)、中耐寒品种(20-4、 52-3)、弱耐寒品种(16P、SC8)做4 ℃低温胁迫处理,测定其幼苗叶片相对电导率、丙二醛、可溶性糖、可溶性蛋白、保护酶活性等7个生理指标,运用主成分分析、隶属函数、相关性分析和逐步回归等方法综合评价木薯种质的耐寒性及筛选耐寒鉴定指标。结果将7个单项生理指标转化为3个相互独立的综合指标,认为测定相对电导率、可溶性糖、丙二醛和过氧化氢酶共4个生理指标可作为准确快速鉴定木薯耐寒性强弱的指标;初步建立起了木薯耐寒性评价体系,发现田间与生理试验结果一致性较高:即参试木薯种质中,最耐寒...

The chilling injury of 18 cassava germplasm resources was classified by natural low temperature in the field. and strong cold-tolerant cultivars (F200), medium-tolerant cultivars (20-4, 52-3), and weak coldtolerant cultivars (16P, SC8) were selected for low temperature (4 ℃) stress treatment. The seedling leaf relative electric conductivity, the malondialdehyde, soluble sugar, soluble protein, protective enzyme activity and other seven physiological indexes were assayed using the method of principal component analysis (PCA), and the membership...