期刊论文

Detection and Genetic Relationship Analysis of Sweet Potato Leaf Curl Virus and Sweet Potato Badnavirus Infecting Sweet Potato Germplasm Resources in Hunan, China

中文

热带作物学报

1000-2561

2021

42

09

2666-2673

10.3969/j.issn.1000-2561.2021.09.031

财政部和农业农村部:国家现代农业产业技术体系 ; 湖南省重点研发计划 ; 湖南省甘薯工程技术研究中心

本校为第一机构

采用PCR技术对2015-2016年从湖南省多个市县采集的180份甘薯种质资源进行两种DNA病毒(sweet potato leaf curl virus,SPLCV和sweet potato badnavirus,SPBV)检测,并以PCR扩增产物序列为基础,对这两种病毒的亲缘关系进行分析。结果表明:(1)180份甘薯种质资源中,22份检测出SPLCV,占总数的12.2%;32份检测出SPBV,占总数的17.8%;8份同时检出SPLCV和SPBV这两种病毒,占总数的3.9%。(2)检出SPLCV的市县有长沙、永州、怀化、邵阳、郴州、常德、娄底、益阳、岳阳和株洲;检出SPBV的市县有长沙、永州、怀化、衡阳和邵阳;检出两病毒复合侵染的市县有怀化、长沙、永州和邵阳。(3)基于病毒全基因组序列分析显示...

180 sweet potato germplasm resources from different regions of Hunan were used to detect sweet potato leaf curl virus (SPLCV) and sweet potato badnavirus (SPBV) during 2015-2016. The genetic relationship of the two viruses was analyzed based on the sequence of PCR amplification products. 22 sweet potato germplasm resources were infected with SPLCV, making up 12.2% of the total, and 32 sweet potato germplasm resources were infected with SPBV, or 17.8% of the total. There were 8 sweet potato germplasm resources infected with both SPLCV and SPPV, ...