Mg-protoporphyrin IX methyltransferase (CHLM) plays a crucial role in chlorophyll biosynthesis and converts Mg-protoporphyrin IX to Mg-protoporphyrin IX monomethyl ester. Previous research has indicated that down-expression of CHLM causes a pale green phenotype and Mg-protoporphyrin IX accumulation in Arabidopsis thaliana plants. Here, we report that a novel missense mutation (G to A transition) in the CHLM gene, results in the Gly59 → Glu amino acid substitution in the corresponding protein of Arabidopsis chlm-4 mutant plants. In silico model...