Sensitive and high throughput quantification of abscisic acid based on quantitative real time immuno-PCR

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成果类型：

期刊论文

作者：

Su, Yi;Li, Wei;Huang, Zhigang;Wang, Ruozhong;Luo, Weigui;...

通讯作者：

Xiao, Langtao

作者机构：

[Huang, Zhigang; Su, Yi; Wang, Ruozhong; Tong, Jianhua; Li, Wei; Luo, Weigui; Liu, Qing; Xiao, Langtao] Hunan Agr Univ, Hunan Prov Key Lab Phytohormones & Growth Dev, Changsha, Hunan, Peoples R China.

[Huang, Zhigang; Su, Yi; Wang, Ruozhong; Liu, Qing; Xiao, Langtao] Hunan Agr Univ, Southern Reg Collaborat Innovat Ctr Grain & Oil C, Changsha, Hunan, Peoples R China.

[Li, Wei] Hunan Acad Agr Sci, Tea Res Inst, Changsha 410125, Hunan, Peoples R China.

通讯机构：

[Xiao, Langtao] H

Hunan Agr Univ, Hunan Prov Key Lab Phytohormones & Growth Dev, Changsha, Hunan, Peoples R China.

语种：

英文

关键词：

qIPCR;ABA;Biotin;Avidin

期刊：

Plant Methods

ISSN：

1746-4811

年：

2018

卷：

期：

页码：

1-10

DOI：

10.1186/s13007-018-0371-y

基金类别：

National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [91317312, 31570372, 9141730003]; National Key Research and Development Program-Seven major crops breeding Project [2016YFD0101803]; Scientific Research Fund of Hunan Provincial Education DepartmentHunan Provincial Education Department [13K065, 15K061]

机构署名：

本校为第一且通讯机构

院系归属：

生物科学技术学院

摘要：

Abscisic acid (ABA) functions as a stress phytohormone in many growth and developmental processes in plants. The ultra-sensitive determination of ABA would help to better understand its vital roles and action mechanisms. We report a new sensitive and high throughput quantitative real time immuno-PCR (qIPCR) method based on biotin–avidin linkage system for ABA determination in plants. ABA monoclonal antibody (McAb) coated on the inner surface of PCR well pretreated with glutaraldehyde. The pre-prepared probe complex, including biotinylated McAb...

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Sensitive and high throughput quantification of abscisic acid based on quantitative real time immuno-PCR

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