期刊论文

Zhi Liu<&wdkj&>Junhua Chen

英文

Science of The Total Environment

0048-9697

2023

881

163465

10.1016/j.scitotenv.2023.163465

We first employed PCB72 as the model target to construct a YES logic gate (Fig. 1). The absence and presence of the target were defined as 0 and 1, respectively. The trigger DNA (DNA2) was blocked by DNA1. In the presence of PCB72, the specific binding between the aptamer (DNA1) and PCB72 will release the blocked DNA2. The free DNA2 thus can be used as the trigger DNA to initiate the CHA cycle amplification reaction. The hairpin probe 1 (H1) can be opened by the hybridization with DNA2 through

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Polychlorinated biphenyls (PCBs) are ubiquitous persistent organic pollutants that cause harmful effects on environmental safety and human health. There is an urgent need to develop an intelligent method for PCBs sensing. In this work, we proposed a logic gate biosensing platform for simultaneous detection of multiple PCBs. 2,3',5,5'-tetrachlorobiphenyl (PCB72) and 3,3',4,4'-tetrachlorobiphenyl (PCB77) were used as the two inputs to construct biocomputing logic gates. We used 0 and 1 to encode the inputs and outputs. The aptamer was used to recognize the inputs and release the trigger DNA. A c...