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Screening of multimeric beta-xylosidases from the gut microbiome of a higher termite, Globitermes brachycerastes

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成果类型:
期刊论文
作者:
Liu, Chunyan;Zou, Gen;Yan, Xing*;Zhou, Xuguo*
通讯作者:
Zhou, Xuguo;Yan, Xing
作者机构:
[Zhou, Xuguo; Liu, Chunyan] Hunan Agr Univ, Coll Plant Protect, Changsha 410128, Hunan, Peoples R China.
[Zou, Gen; Yan, Xing] Chinese Acad Sci, Inst Plant Physiol & Ecol, Shanghai 200032, Peoples R China.
[Zhou, Xuguo] Univ Kentucky, Dept Entomol, Lexington, KY 40546 USA.
通讯机构:
[Zhou, Xuguo] H
[Yan, Xing] C
[Zhou, Xuguo] U
Hunan Agr Univ, Coll Plant Protect, Changsha 410128, Hunan, Peoples R China.
Chinese Acad Sci, Inst Plant Physiol & Ecol, Shanghai 200032, Peoples R China.
语种:
英文
关键词:
*Globitermes brachycerastes;*fosmid library;*glycoside hydrolases;*gut microbiome;*multimeric hemicellulase;*beta-xylosidase
期刊:
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
ISSN:
1449-2288
年:
2018
卷:
14
期:
6
页码:
608-615
基金类别:
High-tech Research and Development Program of China (863)National High Technology Research and Development Program of China [2013AA102806]; Shen-Nong Visiting Scholar Funding Program of Hunan Agricultural University; Kentucky Agricultural Experiment Station [18-08-034]; Hatch fund from the USDA National Institute of Food and Agriculture [1004654, KY008071]
机构署名:
本校为第一且通讯机构
院系归属:
植物保护学院
摘要:
Termite gut microbiome is a rich reservoir for glycoside hydrolases, a suite of enzymes critical for the degradation of lignocellulosic biomass. To search for hemicellulases, we screened 12,000 clones from a fosmid gut library of a higher termite, Globitermes brachycerastes. As a common Southeastern Asian genus, Globitermes distributes predominantly in tropical rain forests and relies on the lignocellulases from themselves and bacterial symbionts to digest wood. In total, 22 positive clones with β-xylosidase activity were isolated, in which 11...

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