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Determination of antibody induction by highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) vaccine: A comparison of two ELISA kits.

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成果类型:
期刊论文
作者:
Ge, Meng;Li, Run-Cheng;Gong, Wenjie*;Tu, Changchun*
通讯作者:
Gong, Wenjie;Tu, Changchun
作者机构:
[Gong, Wenjie; Tu, Changchun; Gong, WJ; Tu, CC; Ge, Meng] Acad Mil Med Sci, Inst Mil Vet Med, Changchun 130122, Jilin, Peoples R China.
[Ge, Meng; Li, Run-Cheng] Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.
通讯机构:
[Gong, WJ; Tu, CC] A
Acad Mil Med Sci, Inst Mil Vet Med, Changchun 130122, Jilin, Peoples R China.
语种:
英文
关键词:
virus antibody;virus vaccine;animal;blood;enzyme linked immunosorbent assay;immunology;pig;porcine reproductive and respiratory syndrome;Porcine reproductive and respiratory syndrome virus;serodiagnosis;vaccination;veterinary medicine;Animals;Antibodies, Viral;Enzyme-Linked Immunosorbent Assay;Neutralization Tests;Porcine Reproductive and Respiratory Syndrome;Porcine respiratory and reproductive syndrome virus;Swine;Vaccination;Viral Vaccines
期刊:
Journal of Veterinary Medical Science
ISSN:
0916-7250
年:
2019
卷:
81
期:
8
页码:
1173-1176
基金类别:
ACKNOWLEDGMENTS. This work was supported by the National Key Research and Development Program of China (2017YFD0500101, 2017YFD0500102).
机构署名:
本校为其他机构
院系归属:
动物医学院
摘要:
Two commercial porcine reproductive and respiratory syndrome virus (PRRSV) antibody ELISA kits (IDEXX and LSI) are currently in extensive use. To determine which kit is more suitable for the evaluation of HP-PRRSV vaccine efficacy, the two kits were used to test 546 serum samples. The agreement between the results was unsatisfactory, with a kappa statistic of 0.681 and a linear correlation coefficient of 0.665. In tests of samples from experimentally vaccinated and PRRSV-negative herds, IDEXX-ELISA identified antibody-positive conversion earlier and showed a higher specificity compared to LSI-...

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