期刊论文

中文

农业现代化研究

1000-0275

2002

23

2

144-147

10.3969/j.issn.1000-0275.2002.02.018

湖南省教委资助项目《去毒桐粕饲料原料标准的制订》（99B0 6）；

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通过研究脱毒桐饼(粕)不同重量和粒度、碱溶液的浓度及pH值、搅拌时间与离心时间以及离心速度等对蛋白质溶解度的影响,确定了测定脱毒桐饼(粕)蛋白质溶解度的适宜条件.结果表明,其适宜的条件是:脱毒桐饼(粕)样品1.5g(±0.001),需置于75ml的pH值为13.8的3%(w/v)KOH溶液中,经磁力搅拌30min,取搅拌液50ml于离心机内离心10min,转速为3 000转/min(Rpm),再取离心上清液15ml,通过凯氏半微量定N法测定其蛋白质含量,最后计算脱毒桐饼(粕)蛋白质溶解度.

The optimum conditions for determining protein solubility(PS) value of detoxification tung cake(meal)with potassium hydroxide solution(KOH) were investigated in the laboratory.The effects of weight,particle size,alkaline concentration(KOH),pH,stirred time,centrifugal rate and centrifugal time on PS value of detoxification tung cake(meal) were determined.The results show that the optimum conditions are as follows:weighing 1.5g(±0.001) of a detoxification tung cake(meal) sample and transferred it into a flask,mixed with 75ml of 3% KOH(pH 13.8),t...