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3H-117, a structural protein of Heliothis virescens ascovirus 3h (HvAV-3h)

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成果类型:
期刊论文
作者:
Zhao, Ying;Yu, Huan;He, Lei;Li, Ni;Huang, Guo-Hua*
通讯作者:
Huang, Guo-Hua
作者机构:
[Li, Ni; Yu, Huan; Huang, Guo-Hua; He, Lei; Zhao, Ying] Hunan Prov Key Lab Biol & Control Plant Dis & Ins, Changsha 410128, Hunan, Peoples R China.
[Li, Ni; Yu, Huan; Huang, Guo-Hua; He, Lei; Zhao, Ying] Hunan Agr Univ, Coll Plant Protect, Changsha 410128, Hunan, Peoples R China.
通讯机构:
[Huang, Guo-Hua] H
Hunan Prov Key Lab Biol & Control Plant Dis & Ins, Changsha 410128, Hunan, Peoples R China.
Hunan Agr Univ, Coll Plant Protect, Changsha 410128, Hunan, Peoples R China.
语种:
英文
关键词:
Ascovirus;Structural protein;HvAV-3h;Phosphatases
期刊:
Virus Genes
ISSN:
0920-8569
年:
2019
卷:
55
期:
5
页码:
688-695
基金类别:
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31872027]
机构署名:
本校为通讯机构
院系归属:
植物保护学院
摘要:
The open reading frame 117 (3h-117) of Heliothis virescens ascovirus 3h (HvAV-3h), which is a conserved coding region present in all completely sequenced ascovirus members, was characterized in this study. By RT-PCR detection, 3h-117 transcription began at 6-h post-infection (hpi) and remained stable until 168 hpi in HvAV-3h-infected Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidae) larvae. In addition, 3h-117 putatively encodes a 21.5-kDa protein (3H-117) predicted to be a CTD-like phosphatase. Western blot analysis using a prepared rabbit polyclonal antibody specific to 3H-117 showed th...

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