期刊论文

Preparation of non-extractable polyphenols from Kumquat and its immunomodulatory effect in RAW 264.7 macrophage cells

中文

精细化工

1003-5214

2021

38

05

973-980

10.13550/j.jxhg.20200956

国家自然科学基金项目（31671858）； 湖南省自然科学基金项目（2019JJ40114）； 湖南省教育厅重点项目（18A108）； 长沙市重点研发计划项目（kq1801016）；

本校为第一机构

以萃取游离多酚后的金柑果渣为材料,采用高压脉冲电场（PEF）进行处理,提取其中的结合多酚（NEPP）。在单因素（场强、脉冲数和液料比）实验基础上,以金柑NEPP含量为响应值,通过响应面法对金柑NEPP提取条件进行优化;之后应用RAW 264.7巨噬细胞模型,分别采用MTT法、中性红比色法以及Griess法,测定金柑NEPP处理后的RAW 264.7细胞生长情况、吞噬活性和释放NO能力,以评价其免疫活性。结果表明,金柑NEPP的提取工艺回归模型显著,拟合性良好,并预测金柑NEPP含量为1.6728 mg GAE/g DW（即每克金柑干重所含有的没食子酸当量）;优化后的PEF提取条件为:脉冲数250次,场强11.8 kV/cm,液料比0.34∶1（L∶g）。...

Non-extractable polyphenol (NEPP) was extracted from the pomace of post-citrus polyphenols by high intensity pulsed electric field (PEF). On the basis of single-factor experiments (field intensity, pulse number and liquid-solid ratio), the extraction condition of NEPP in Kumquat was optimized by response surface method using NEPP content as response value. MTT assay, neutral red colorimetric assay, and Griess assay were used to test the growth, phagocytic activity, and NO release of RAW 264.7 macrophage cells after NEPP treatment to evaluate th...