An investigation into the possible substitution of Clostridium autoethanogenum protein (CAP) for soybean meal (SBM) in the diet of grass carp (Ctenopharyngodon idellus) was carried out. During a 56-day feeding trial, 750 grass carp were fed five experimental diets with different levels of CAP: SBM (control group), CAP25, CAP50, CAP75, and CAP100, corresponding to dietary SBM replacements levels of 0%, 25%, 50%, 75%, and 100%. The results showed that dietary CAP significantly increased FW, WGR, and PER in CAP25 group (P < 0.05), but complete substitution showed the opposite trend (P < 0.05). Th...