期刊论文

Application of fluorescent staining buffer in test for trace protein in SDS-PAGE

Yu, X.-L.

中文

中国生物制品学杂志

1004-5503

2019

32

7

803-805

10.13200/j.cnki.cjb.002683

国家自然科学基金项目(31672539).

本校为第一且通讯机构

目的探讨立显蛋白染色液在SDS-PAGE中检测微量蛋白的应用效果。方法制备SDS-PAGE胶,采用立显蛋白染色液及普通考马斯亮蓝(Coomassie brilliant blue,CBB)染色液,检测定量的牛血清白蛋白(bovine serum albumin, BSA)的敏感度及猪细小病毒VP2蛋白、猪蓝耳病病毒N蛋白、猪丹毒杆菌Fe蛋白和猪伪狂犬病病毒gB蛋白基因工程纯化样品的纯度。结果采用立显蛋白染色液染色可识别蛋白质含量为2. 5 ng的蛋白条带,敏感性高于普通CBB染色40倍。4种蛋白纯化样品经CBB染色后检测纯度均较高,但经立显蛋白染色液染色后,4种纯化蛋白样品均检测出杂蛋白,测定的纯度均有所下降,其中VP2及gB蛋白的纯度有较大幅度下降(分...

Objective To investigate the application of fluorescent staining buffer in tesl for trace protein in SDS-PAGE. Methods SDS-PAGE gel was prepared, with which the sensitivities of fluorescent staining buffer and Coomassie brilliant blue (CBB) in determination of quantitative bovine serum albumin (BSA) were compared. On the basis of this, the purities of porcine parvovirus VP2 protein, porcine reproductive and respirator)-syndrome virus N protein, Erysipelas erysipelas Fe protein and porcine pseudorabies virus gB protein determined with fluorescen...