There are only one or two different genes between near isogenic lines, which are useful to reduce a lot of unnecessary work. So they are widely used in crop breeding, molecular markers and gene mapping. Rapeseed is the largest oil crops in China, and Brassica napus L. is the widest type of rapeseed. The application of construction of Brassica napus L. near isogenic lines and the research of stress tolerance, molecular marker and proteomics studies were summarized in this paper. With the development of new construction technologies, ne...