期刊论文

中文

作物杂志

1001-7283

2016

4

36-40

10.16035/j.issn.1001-7283.2016.04.006

国家863计划:强优势油菜杂交种的创制与应用（2011AA10A104）；

本校为第一机构

本研究利用聚丙烯酰胺凝胶垂直板电泳技术,对油菜新型胞质雄性不育系1193A和保持系1193B花蕾发育过程中的过氧化物酶（POD）、三磷酸腺苷（ATP）酶和酯酶（EST）3种同工酶进行分析。结果表明,在花蕾生长发育的不同时期不育系与保持系的同工酶谱带数及表达活性之间均存在差异。在花蕾发育后期,不育系1193A的POD同工酶中迁移率最快的一条酶带活性明显强于保持系1193B;不育系1193A的ATP和EST同工酶活性都是随着花蕾发育逐渐减弱的,且在花蕾发育后期都出现了酶带的缺失。

In this study, the POD, ATP and EST isozymes from new cytoplasmic male sterile line 1193 A and maintain line 1193 B of Brassica napus L. in the development of flower buds were analyzed by polyacrylamide gel electrophoresis. The results showed that there were differences in the number of isozyme bands and expression between the male sterile line and the maintain line in the different stages of the growth and development of the flower buds. In later developmental stage,POD isozymes bands activity of the fastest mobility in the male sterile line 1193 A was significantly stronger than that of the ...