期刊论文

Residue Analysis of Pymetrozine and Clothianidin in Citrus and Soil

中文

农药

1006-0413

2019

58

6

448-450

10.16820/j.cnki.1006-0413.2019.06.015

本校为第一机构

[目的]建立了柑橘和土壤中吡蚜酮、噻虫胺的高效液相色谱-串联质谱(HPLC-MS/MS)同时检测分析方法。 [方法]采用外标法进行定量,柑橘和土壤中的吡蚜酮和噻虫胺先经0.1%氨水乙腈溶液振荡提取,然后采用中性氧化铝、无水硫酸钠净化,最后经HPLC-MS/MS检测。[结果]在0.01、0.05、0.1、0.5、1.0 mg/kg标准工作溶液浓度下,吡蚜酮相关系数0.999,噻虫胺相关系数0.9999;在0.05、0.5、1 mg/kg添加水平下,吡蚜酮在土壤、果肉以及全果中的平均回收率为89%~108%,其相对标准偏差(RSD)在5%~20%范围内;噻虫胺的平均回收率达到92%~109%,其相对标准偏差为3%~16%。吡蚜酮与噻虫胺在试验中的检出限(LOD)均为0.01 mg/kg...

[Aims] A practical and effective method was established and verified for determination of pymetrozine and clothianidin in citrus and soil through high performance liquid chromatography equipped mass spectrometry (HPLC-MS/MS). [Methods] The external standard method was used for quantification. Pymetrozine and clothianidin in citrus and soil were extracted with 0.1% ammonia acetonitrile solution, then purified by neutral alumina and anhydrous sodium sulfate, and finally detected by HPLC-MS/MS. [Results] At 0.01-1.00 mg/L, the correlation coefficient of pymetrozine was R~2=0.999, the correlation ...