期刊论文

Development of Bacillus subtilis JN005 WP and Its Control Effect on Rice Blast

中文

农药

1006-0413

2019

58

6

415-419

10.16820/j.cnki.1006-0413.2019.06.006

国家重点研发计划项目(2016YFD0300700) 中央财政专项(2014ZX0800102B) 公益性行业(农业)科研专项(201203014).

本校为第一机构

[目的]为了研制一种对水稻稻瘟病具有良好防治效果的生防菌剂。[方法]以枯草芽孢杆菌JN005为材料,采用单因素法和正交试验法筛选出对菌株JN005活性影响最小的载体和助剂及其配比。采用喷雾接种法测定了不同时期喷施枯草芽孢杆菌JN005可湿性粉剂对盆栽水稻品种湘晚籼12号苗叶瘟病的防治效果。[结果]可湿性粉剂的最佳配方为枯草芽孢杆菌JN005菌冻干粉10%,茶皂素5%,Yus-WP1 5%,羧甲基纤维素(CMC)1%,白炭黑79%,该可湿性粉剂活菌含量为1.98×10~(10) cfu/g,细度98%,润湿时间112 s,悬浮率77.24%,pH值7.2,热贮稳定性82.83%,干燥减量1%。当水稻长至3叶1心,接种稻瘟病菌分生孢子悬浮液(浓度2×10~5 cfu/m...

[Aims] In order to develop a biocontrol agent with good efficacy on rice blast caused by Magnaporthe oryzae. [Methods] Bacillus subtilis JN005 was used as material, carriers and auxiliaries with the least influence on the activity of strain JN005 were screened by single factor and orthogonal test. The control effect of B. subtilis JN005 WP on rice seeding leaf blast disease of potted rice variety Xiangwanxian No.12 was determined by spray inoculation. [Results] The optimum formula of the wettable powder was as follows: 10% of freeze-dried powde...