This work was supported by the Natural Science Foundation of China (31401637) and Science Funding of The Education Department of Hunan Province (15K062). The funders had no role in the study design, data collection and analysis, decision to publish or preparation of the manuscript.
机构署名：
本校为第一且通讯机构
院系归属：
生物科学技术学院
摘要：
BACKGROUND: Mannanases are important enzymes which are widely used as a tool in agriculture and food industries. To improve the performance of mannanase Man23, a mutant library was created with rational design, and mutations were introduced on loops around the catalytic region. The Brevibacillus brevis B16 cell-free system which was created in this experiment provided the ability to express the mutant library efficiently. The activities of mutants were measured with a multi-volume spectrophotometer. RESULTS: The mutant Man1606 gained from this ...
