期刊论文

Prokaryotic expression and transcription analysis of Heliothis virescens ascovirus 3h encoded 3h-38 gene

中文

环境昆虫学报

1674-0858

2021

43

05

1113-1121

10.3969/j.issn.1674-0858.2021.05.4

国家自然科学基金（32070168）；

本校为第一机构

烟芽夜蛾囊泡病毒3h株（Heliothis virescens ascovirus 3h, HvAV-3h）作为一种极具生防潜力的环状双链DNA昆虫病毒,自被分离后,对其基因的特性和相关蛋白功能研究从未间断。本研究通过生物信息学预测3H-38蛋白序列发现其131～150氨基酸位置有一段由胞外向胞内的跨膜区序列,N端23～121氨基酸位置有一个BRO家族结构域,和其同源蛋白的序列对比发现3H-38与烟芽夜蛾囊泡病毒3i株（Heliothis virescens ascovirus 3i, HvAV-3i）和烟芽夜蛾囊泡病毒3j株（Heliothis virescens ascovirus 3j, HvAV-3j）编码的同源蛋白3I-40和3J-43相似性高达85%以上。进一步通过RT-PCR克隆、构建pET-28a-38原核表达载体...

As a large double stranded DNA insect virus,Heliothis virescens ascovirus 3h (HvAV-3h) has high potential to develop into biological control agent,and the studies on the gene characteristics and related protein functions of HvAV-3h has been carried on since it was isolated. In this study,the putative protein sequence of 3H-38 was predicted to contain a transmembrane region and a bro family domain (23 ~ 121 amino acid in the N-terminal). Compared with the homologous proteins in other ascovirus isolates,a high identity above 85% was found between 3H-38 and 3I-40 (encoded by HvAV-3i) and 3J-43 (e...