Until now, only the complete mitochondrial genome (mtDNA) of Pelecanus occidentalis and Pelecanus crispus have been sequenced. In order to explore the characteristics of mtDNA of the two Pelecanidae species and analyze their phylogenetic relationship, the two mtDNA were compared with Ciconia ciconia as reference. The mtDNA of the P. occidentalis and P. crispus were determined with the size of 17, 286 bp and 16, 131 bp, respectively. Both sequences contain 13 protein-coding genes, 22 tRNA genes, and two rRNA genes. Gene of P. occidentalis and P. crispus are in the same arrangement and show posi...