期刊论文

中文

中国卫生检验杂志

1004-8685

2013

23

16

3195-3197

国家“十二五”科技支撑计划项目（2011BADl0800） 湖南省教育厅科学研究项目（11C0680）

本校为第一机构

目的 建立超声波辅助提取-高效液相色谱(HPLC)检测的方法,同时分离分析猕猴桃果实中的3种有机磷农残.方法 用正己烷:丙酮(80％,V∶V)超声提取后,经HPLC-UV检测,采用基体匹配法校准曲线有效降低了样品的基体效应.结果 3种有机磷农药分离较好,其线性范围为0.10 μml ～1.00 μg/ml;检出限(LOD)介于0.005 μg/ml～0.014 μg/ml;加标回收率(R)在76.30％～ 95.00％之间.结论 该方法简便易行,且精密度高,准确度好,具有很大的实用价值.

Objective To establish a method by ultrasonic - assisted extraction - HPLC for separation and analysis of three kinds of organophosphorous pesticides in kiwi fruit simultaneously. Methods The targeted analytes were extracted by n - hex- ane ：acetone （80%, V：V） and detected by HPLC. The matrix matching method was applied to calibrate the linearity of the curve to reduce the substrate effect. Results The three kinds of organophosphorous pesticides were separated well in the linear range of 0.10 p,g/ml-1.00 μg/ml, the limits of detection （LO...