Characterization of Oesophagostomum spp. from pigs in China by PCR-based approaches using genetic markers in the internal transcribed spacers of ribosomal DNA

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成果类型：

期刊论文

作者：

Lin, Rui-Qing;Zhu, Xing-Quan*;Wei, Dong-Xia;Deng, Yan;Liu, Wei;...

通讯作者：

Zhu, Xing-Quan

作者机构：

S China Agr Univ, Coll Vet Med, Guangzhou 510642, Guangdong, Peoples R China.

Jiangsu Anim Husb & Vet Coll, Dept Vet Sci, Taizhou 225300, Jiangsu, Peoples R China.

Guangzhou Airport Entry Exit Inspect & Quarantine, Gen Lab Inspect & Quarantine, Guangzhou 510406, Guangdong, Peoples R China.

Hunan Agr Univ, Coll Anim Sci & Technol, Changsha 410128, Peoples R China.

Sun Yat Sen Univ, Sch Life Sci, Guangzhou 510275, Guangdong, Peoples R China.

通讯机构：

[Zhu, Xing-Quan] S

S China Agr Univ, Coll Vet Med, 483 Wushan St, Guangzhou 510642, Guangdong, Peoples R China.

语种：

英文

关键词：

Polymerase Chain Reaction Product;Internal Transcribe Spacer;Restriction Fragment Length Polymorphism Analysis;Dongting Lake;SSCP Analysis

期刊：

Parasitology Research

ISSN：

0932-0113

年：

2007

卷：

101

期：

页码：

351-356

DOI：

10.1007/s00436-007-0498-7

机构署名：

本校为其他机构

院系归属：

动物科学技术学院

摘要：

In the present study, samples of Oesophagostomum spp. collected from pigs from different geographical localities in mainland China were characterized genetically by polymerase chain reaction-linked single-strand conformation polymorphism (PCR-SSCP) and restriction fragment length polymorphism (PCR-RFLP) techniques using genetic markers in the internal transcribed spacers (ITS) of nuclear ribosomal DNA (rDNA). The second internal transcribed spacer (ITS-2) was amplified from 51 individual nodule worms by PCR, and the amplicons were analyzed by SSCP. With the exception of slight microheterogenei...

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Characterization of Oesophagostomum spp. from pigs in China by PCR-based approaches using genetic markers in the internal transcribed spacers of ribosomal DNA

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