期刊论文

DeveIopmentaI toxicity of trichIoroisocyanuric acid on zebrafish embryos and Iarvae

Liu, S.-C.

中文

中国药理学与毒理学杂志

1000-3002

2017

31

7

736-741

10.3867/j.issn.1000-3002.2017.07.006

深圳市科技研发资金项目（CYJ2014041017-1018510） 深圳市卫计委重点学科建设能力提升项目（201506064） 深圳市科技研发资金学科布局项目（JCYJ20160328144536436）

本校为第一且通讯机构

目的探讨新型消毒剂三氯异氰尿酸(TCCA)对斑马鱼胚胎及幼鱼的发育毒性。方法选择受精后2 h的斑马鱼胚胎进行染毒:①将胚胎置于含有TCCA 50,100,150,200,250和300 mg·L~(-1)的培养液中暴露48 h,检测半数致死浓度(LC_(50))。②将胚胎置于含有TCCA 0,10.4,20.8和41.7 mg·L~(-1)的培养液中暴露96 h,分别于暴露后48,72和96 h检测胚胎死亡率、畸形率和心率,采用羟胺法于暴露后2,4和6 h检测胚胎组织超氧化物歧化酶(SOD)的活性,HE染色观察暴露后7 d幼鱼头部组织病理结构。结果TCCA暴露48 h,斑马鱼胚胎LC_(50)为166.9 mg·L~(-1)。与正常对照组相比,暴露后96 h,TCCA 41.7 mg·L~(-1)组斑马鱼胚胎死亡率...

OBJECTIVE To investigate the developmental toxicity of a new disinfectant trichloroisocyanuric acid (TCCA) on zebrafish embryos and larvae. METHODS Zebrafish embryos of 2 h post fertilization (hpf) were exposed to TCCA: ① The embryos were exposed to a culture medium containing TCCA 0, 50, 100, 150, 200, 250 and 300 mg·L~(-1) for 48 h before 50% lethal concentration (LC_(50)) was calculated. ② The embryos were exposed to a culture medium containing TCCA 0, 10.4, 20.8 and 41.7 mg ·L~(-1) for 96 h. The mortality, malformation rate and heart ra...