期刊论文

中文

中国饲料

1004-3314

2019

23

64-68

10.15906/j.cnki.cn11-2975/s.20192316

国家自然科学基金项目(31902196) 湖南省自然科学基金项目(2019JJ50268) 湖南农业大学青年基金项目(17QN25)。

本校为第一机构

为探讨浒苔多糖(EP)对产肠毒素大肠杆菌(ETEC)感染小鼠肠道黏膜的保护作用及最适添加剂量,试验分为空白对照组、ETEC模型组、300 mg/L EP+ETEC组、600 mg/L EP+ETEC组和1000 mg/L EP+ETEC组.所有小鼠饲喂维持饲粮,对照组饲喂饮用水,EP试验组在饮用水中添加相应剂量浒苔多糖.造模采用灌胃ETEC方法,并对小鼠血清炎性因子及空肠相关蛋白丰度进行检测.结果 表明:添加600 mg/L剂量的浒苔多糖使感染后小鼠血清中TNF-α及IL-6水平显著降低30.22％、56.70％(P＜ 0.05),Claudin-1在小鼠空肠中的表达丰度显著上调80％(P＜0.05).结合空肠NF-κB信号通路检测结果表明,浒苔多糖可缓解ETEC感染导致的小鼠肠道黏...

This study investigated the protective effect of Enteromorpha polysaccharide(EP)on intestinal mucosa of mice infected with enterotoxigenic Escherichia coli(ETEC)and the optimal dosage.There were five treatments in this study,namely control group,ETEC group,300 mg/L EP+ETEC group,600 mg/L EP+ETEC group and 1000 mg/L EP+ETEC group.All mice were fed with basal diet.The EP group was given a corresponding dose of EP,while the control group was given with drinking water.Intestinal inflammation was infected with ETEC,and serum inflammatory factors and...