期刊论文

Microsporogenesis and Male Gametogenesis in Diploid Miscanthus sinensis

中文

西北植物学报

1000-4025

2019

39

5

770-775

10.7606/j.issn.1000-4025.2019.05.0770

国家自然科学基金(31471557).

本校为第一机构

该实验通过采集天然二倍体芒不同发育时期的幼穗,进行卡宝品红染色压片和石蜡切片制作,观察芒的花药发育过程,为芒的生殖生物学及其系统发育研究奠定理论基础。结果表明:(1)天然二倍体芒雄蕊3枚,雄蕊花药四室,花药壁由4层细胞组成,从外向内依次是表皮、药室内壁、中层、绒毡层,花药壁发育属于基本型;花药成熟时,中层和绒毡层降解消失,或者仅存痕迹,只剩表皮和纤维层细胞,但此时可观察到部分绒毡层降解延迟现象。(2)花粉母细胞减数分裂为连续型,成熟花粉粒为3-细胞型;花粉母细胞减数分裂后期Ⅱ出现染色体分裂不同步现象。(3)雄配子体发育过程中,同一花粉囊的花粉粒发育不同步;雌、雄配子体发育也...

The anther development process of natural diploid Miscanthus sinensis has been observed by carbol fuchsin staining and paraffin sectioning, after collecting young spikes of natural diploid M. sinensis at different stages. This experiment lay the theoretical foundation for reproductive biology and phylogeny of M. sinensis . The results as follows:(1) in natural diploid M. sinensis , there are 3 stamens and the anther is 4 sporangiates, which wall is composed of 4 layers: epidermis, endothecium, middle layer and tapetum. The development of anther...