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Rapid annotation and structural characterization of saponins in the active fraction of Albizia julibrissin by high-performance liquid chromatography coupled with quadrupole time of flight mass spectrometry based on accurate mass database

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成果类型:
期刊论文
作者:
He, Yanfei;Ni, Tiantian;Liu, Zhaoying;Ye, Yiping;Sun, Hongxiang*
通讯作者:
Sun, Hongxiang
作者机构:
[Sun, Hongxiang; Ni, Tiantian; He, Yanfei] Zhejiang Univ, Coll Anim Sci, Lab Nat Drug, Yuhangtang Rd 866, Hangzhou 310058, Zhejiang, Peoples R China.
[Liu, Zhaoying] Hunan Agr Univ, Coll Vet Med, Hunan Engn Res Ctr Vet Drug, Changsha, Hunan, Peoples R China.
[Ye, Yiping] Zhejiang Acad Med Sci, Inst Materia Med, Hangzhou, Zhejiang, Peoples R China.
通讯机构:
[Sun, Hongxiang] Z
Zhejiang Univ, Coll Anim Sci, Lab Nat Drug, Yuhangtang Rd 866, Hangzhou 310058, Zhejiang, Peoples R China.
语种:
英文
关键词:
Accurate mass database;Albizia julibrissin saponin;characterization;high-performance liquid chromatography;tandem mass spectrometry
期刊:
JOURNAL OF SEPARATION SCIENCE
ISSN:
1615-9306
年:
2019
卷:
42
期:
18
页码:
2922-2941
基金类别:
National Key R&D Program of China [2017YFD0501505]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31272597, 31472229, 31772783]; Zhejiang Provincial Natural Science Foundation of ChinaNatural Science Foundation of Zhejiang Province [LZ13C180001]; Dabeinong Funds for Discipline Development and Talent Training in Zhejiang University
机构署名:
本校为其他机构
院系归属:
动物医学院
摘要:
The purified active fraction of Albizia julibrissin saponin was proved to be a promising adjuvant candidate for vaccine. In this study, a simple, convenient, and practical strategy was established for characterizing the saponins in this purified active fraction. The personal accurate mass database including chemical structure, molecular formula, and theoretical mass was first constructed by collecting 110 reported known saponins from genus Albizia species. The raw data was obtained by high-performance liquid chromatography coupled with quadrupole time of flight mass spectrometry. The potential...

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