Drought represents a prevalent abiotic stress in terrestrial plants, frequently impairing crop growth and yield. In this paper, we characterized the functional role of OsNAC25, a member of the NAC transcription factor family, in drought tolerance. OsNAC25 was predominantly localized in both the cytoplasm and nucleus, with its expression being markedly induced under drought conditions. Under severe drought stress, the overexpression of OsNAC25 rice exhibited decreased malondialdehyde (MDA) levels, attenuated oxidative damage, and improved survival rate during the vegetative growth stage. The tr...